ONE-POT PREPARATION OF POLYETHLYENIMINE/SILICA HYBRIDE NANOPARTICLES AS SERUM-RESISTANT GENE DELIVERY VECTORS:TRANSFECTION AND INTRACELLULAR TRAFFICKING

摘要

Novel organic/inorganic hybrid silica nanoparticles with size below 200 nm were one-pot prepared via Michael addition between 3-methacryloxypropyltrimethoxysilane (MPTMS) and polyethylenimine (PEI), followed by hydrolysis and polycondensation of silaxanes. In vitro transfection assay demonstrated that the transfection efficiencies of the nanoparticles were superior to PEI (25 kDa) in the presence of 10% serum.