AN APPARATUS AND METHOD FOR IN VIVO INTRACELLULAR TRANSFECTION OF GENE, SIRNA, SHRNA VECTORS, AND OTHER BIOMEDICAL DIAGNOSTIC AND THERAPEUTIC DRUGS AND MOLECULES FOR THE TREATMENT OF ARTHRITIS AND OTHER ORTHOPEDIC DISEASEAS IN LARGE ANIMALS AND HUMANS

摘要

An apparatus for in vivo intracellular transfection of gene, siRNA, shRNA vectors, and other biomedical diagnostic and therapeutic drugs and molecules for the treatment of arthritis and other orthopedic diseases in large animals and humans includes: a source of low voltage, short duration pulses in long duration bursts (LSEN); an electrode mesh system coupled to the source for generating distributed electric field network into a joint, including bones, cartilages, and related tissues; and means for transfecting the gene, siRNA, shRNA vectors, and other biomedical diagnostic and therapeutic drugs and molecules into a joint. The electrode mesh system includes alternatively arranged negative and positive electrodes in a first array which is capable of being inserted into a joint cavity, and either an alternatively arranged negative and positive or an all negative second electrode array which is positioned outside of the joint and in directly contact with overlying skin.