Micropatterned Biorecognition Surfaces on Nonbiofouling Polymer by Living Radical Photopolymerization for High Sensitivity Biosensing

摘要

Preparation of nonbiofouling surface and micropatterned biorecognition layer over nonbiofouling for highly sensitive microarray biosensors, was conducted by constructing biocompatible poly(2-methacryloyloxyethyl phosphorylcholine(MPC)) brushes on polymer substrates based on living radical polymerization using macrophotoiniferters comprised of 2-ethylhexyl methacrylate (EHMA) and 4-vinylbenzyl N, N-diethyldithiocarbamate (VBDC). Protein adsorption on these modified surfaces significantly reduces compared to that on polymeric substrates, and the micropatterning consisted of poly(MPC) brush and biorecognition layer can be easily done by applying photomask with high fidelity.