Cold-rolling emulsion wastewater (CREW) is characterized by high oil content, complex organic components and certain toxicity. By traditional physical and chemical methods, it could not be completely degraded at low cost. In order to optimize treatment process of CREW, a highly effective strain (Pseudomonas fluorescens) was isolated and combined with bioaugmentation technology, biofilm and fluidized bed technology to treat CREW. After 78 days of the system operation, the removal rate of COD and oil reached 94% and 97%. COD and oil content of the effluent were stably below 70mg/L and 4.7mg/L, which met the first order of the national discharge standard (GB8978-1996). The result of high throughput 16SrRNA sequencing showed there were 23 genera of bacteria in the biofilm community of the reactor. This microbial community structure could effectively degrade COD and oil in CREW. At phylum level, y-proteobacteria, Firmicutes and p-proteobacteria widely existed in carrier and were the main members of pollutant degradation.
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