The growth differences between Leptolyngbya HS-16 isolated from Gunung Pancar hot spring (69 °C) and Leptolyngbya HS-36 isolated from Maribaya hot spring (42 °C) incubated in 20 °C and 50 °C

摘要

The growth differences between cyanobacteria strains Leptolyngbya HS-16 and HS-36, which were incubated in temperatures 20 °C, and 50 °C had been studied. Those strains were isolated from Gunung Pancar (Leptolyngbya HS-16) and Maribaya (Leptolyngbya HS-36) hot springs which were in West Java, Indonesia. The water temperature of habitats was 69 °C (Gunung Pancar) and 42 °C (Maribaya). Those strains were grown in batch culture for 7 days in BG-11 medium. Biomass weight during growing of strains Leptolyngbya HS-16 and HS-36 was the parameter of the growth. Observations were done from day-0 (t_0) until day-7 (t_7). The results showed that there were differences in growth curve of those strains. Biomass weight in day-2 (t_2) of Leptolyngbya HS-16 incubated in 20 °C was higher than biomass weight in day-0 (t_0), however biomass weight in seven days of Leptolyngbya HS-16 incubated in 50 °C was lower than biomass weight of day-0 (t_0). Biomass weight of Leptolyngbya HS-36 incubated in 20 °C was lower than biomass weight of day-0 (t_0). Biomass weight in day-3 (t_3) of Leptolyngbya HS-36 incubated in 50 °C higher than biomass weight in day-0 (t_0). Leptolyngbya HS-16 and Leptolyngbya HS-36 that were incubated in 20 °C and 50 °C went through adaptation (lag) phase. The color of Leptolyngbya HS-16 culture, which was incubated in 20 °C, changed from emerald green color (t_0) into leaf green color (t_7). It also happened at the color of Leptolyngbya HS-36 culture, which was incubated in 50 °C it changed from brown ochre color (t_0) to nougat color (t_7). Growth curve data showed the highest rate of growth in lag phase of Leptolyngbya HS-16 was in 20 °C and Leptolyngbya HS-36 was in 50 °C, but their color of cultures were changed because of the metabolic reaction.