DNA barcoding to identify the genetic diversity of gabus Sentani fish (Oxyeleotris heterodon, Weber 1907) at Putali Gulf Sentani Lake

摘要

Gabus Sentani fish (Oxyeleotris heterodon, Weber 1907) is a native fish at Sentani Lake. The fish has an important economic value for the people who live in the banks of Lake Sentani. The high exploitation made the population decreasing and the status of this fish has not been evaluating by the IUCN. The decreasing population of gabus Sentani fish will make changes in genetic variation and will further lead to the occurrence of inbreeding. The purpose of this study was to determine the genetic variation in fish populations of Gabus Sentani fish in the Putali Gulf-Sentani Lake. The sample area is in the Gulf of Putali - Sentani Lake. It is because the gulf is one of the candidates for asylum fisheries habitat in the Sentani Lake. The analysis of genetic variation was conducted by using DNA Barcoding with Cytochrome Oxidase Gene Sub Unit 1 (COX 1). DNA amplification was obtained along 648 bp. Genetic distance ranges from 0,000 to 0,027. The phylogenetic tree formed forms three main groups. DNA Barcoding is effective for identifying the genetic diversity of gabus Sentani fish (Oxyeleotris heterodon, Weber 1907) located in Putali Gulf - Sentani Lake.