POTENTIAL OF GHRELIN PROTEIN ORIGINATED FROM PLANT AS ENERGY BALANCE SETTINGS FOR FEED EFFICIENCY

摘要

The purpose of this study was to determine the molecular weight of ghrelin protein as a basis to determine its amino acid sequence and then to make the protein's synthetics with a function to regulate energy balance in broilers. Samples were isolated from gastrointestinal tract and brain tissue from the broilers and examined by SDS Page, Western blot and MALDI-TOP. Based on the results, it can be concluded that the molecular weight of the protein ghrelin is a 44kDa with the amino acid sequence mflrvil. By knowing the molecular weight and amino acid composition of the ghrelin protein, we can produce synthetic ghrelin protein to regulate energy balance of the broilers. Exploration of natural ingredients derived from salad leaves, spinach leave, and cassava leaves showed the presence of ghrelin protein.