Kenikir Leaves (C. caudatus) Extract Antibacterial Test Toward The Growth Of Staphylococcus aureus In-Vitro

摘要

The skin wound could cause bacterial infection if not to be treated. One of the cause is Staphylococcusaureus bacteria. Kenikir leaves has been used to be consumed and for traditional medication. This research aims to know the antibacterial activity of Kenikir leaves extract, to test the difference of antibacterial activity through different extraction method of Kenikir leaves and also to know minimum inhibitory concentration. The method that was used to test antibacterial activity was Kirby-Bauer method. Antibacterial activity was characterized by the presence of clear zone around paper discs which were called an inhibitory zone. Method to test a minimum inhibitory concentration were solid dilution. The research used two methods of extraction, namely maceration with ethanol solvent and mashed with aquades solvent. Extract concentrations that were used were 30%, 45%, 60%. Result showed that Kenikir leaves extracts had antibacterial activity. The results of antibacterial activity of the extracts showed that there was no difference between the two extraction method. Mashed extract of Kenikir leaves had a smallest inhibiton zone at concentration of 30% which were 6,76 mm and the largest was 7,58 mm at concentration of 60%. Ethanol extract of Kenikir leaves at concentration of 30% had an inhibiton zone of 7,25 mm and concentration of 60% had 8,59 mm. Minimum Inhibitory Concentration of antibacterial activity was not obtained. Kenikir leaves extract belongs to bacteriostatic group because of the compound could only inhibit the growth of bacteria.