Age-Related Senescence of Rat Bone Marrow-Derived Mesenchymal Stem Cells

摘要

To study whether bone marrow-derived mesenchymal stem cells (MSCs) from aged rat present cell senescence. In the present study, cell morphology were observed, and cell proliferation and cell cycle were analyzed. The natural senescence of MSCs from aged rat was testified by investigation of SA-β-gal activity, ROS levels, DNA damage, telomerase activity and senescence-associated factors. Our results demonstrated that MSCs from aged rat showed senescent morphology and cell growth slowed down. SA-β-gal activity was elevated, the intracellularROS was enhanced, cellular DNA was severely damaged, telomerase activity was declined, and the expression of senescence-associated factors p16~(INK4A) and p21~(WAF1/CIP) were upregulated. Taken together, our findings reveal that MSCs display biological changesand natural senescence in the process of physiological individual aging.