Preparation of Carica Papaya Lipase and its Catalytic Resolution on Flurbiprofen Methyl Ester

摘要

Carica papaya lipase (CPL) stored in the crude papain was explored as enantioselective biocatalys for the hydrolysis resolution of flubiprofen methyl ester. According to its natural properties, 13 percent of carica papaya lipase was obtained from crude enzyme extraction where the activity of the lipase was 90U/g. The optimum hydrolysis conditions of carica papaya lipase were as below, the lyophilization time was 8 hours, the reaction temperature was 35°C, and the pH was 7.0. Based on the hydrolysis catalyzed by carica papaya lipase, it was found that carica papaya lipase has a good tolerance to the low polar organic solvents. On the basis of single factor experiments the optimal conditions was built when the reaction was carried out in the iso-octane with 10mg/mL of flurbiprofen methyl ester, 70mg of carica papaya lipase, 5% of the buffer, 150rpm of the stirring speed, at 35°C for 7 days. The maximum conversion was 14%, and the enantiomer excess of product was 87%. Comparison of two methods for flubiprofen resolution indicated that the second resolution method can get a higher optical purity of flurbiprofen than that of the single lipase resolution method.