Effect of Solvent and Drug Impregnation Techniques on Total Drug Content in Rifampicin Impregnated Hydroxyapatite for Localized Bone Tuberculosis Treatment

摘要

Rifampicin drug was experimentally loaded into 3D printed porous hydroxyapatite using two types of solvents (methanol or N-methyl-2-pyrrolidone) and various solution impregnation techniques aiming to maximize the total drug content in the samples. All vacuum assisted impregnations gave greater rifampicin content in the sample than that of atmospheric immersion. For similar vacuum assisted impregnation technique, the use of methanol could produce greater amount of drug in the sample than using N-methyl-2-pyrrolidone. For each solvent, the loading technique which gave maximum drug content was different. 2_step vacuum loading technique could impregnate the greatest amount of drug in the hydroxyapatite sample when using methanol as a solvent while one step vacuum loading technique with 10 % solution level (l_step_10) gave the greatest amount of the impregnated drug when using N-methyl-2-pyrrolidone as a solvent. These differences were related to the evaporation rate of the solvent and the degree of concentrated drug on the surface of the samples.