Bioaerosol Emissions and Detection of Airborne Antibiotic Resistance Genes from a Wastewater Treatment Plant

摘要

This study was designed to investigate the bioaerosol emissions and airborne antibiotic resistant genes from a wastewater treatment plant (WWTP) in Beijing, China. Here, the biological contents from air samples of 12 sampling sites shown in Fig 1, office building and the boundary of plant in downwind area) and 4 downwind sites (200 m, 1 km, 5 km and 10 km away from the boundary of plant, respectively) were studied by using the Reuter Centrifugal Sampler High Flow (RCS) and high-throughput gene sequence. The viable bioaerosol concentrations of 7 intra-plant sites were also monitored using an ultraviolet aerodynamic particle sizer (UV-APS) for 30 min. Here seven resistant genes and three integrons from both air and water samples collected from the plant were screened by PCR coupled with gel electrophoresis.