Cloning and Characterization of the TMEPAI Gene Promoter

摘要

TMEPAI was originally identified as a highly androgen-induced gene by serial analysis of gene expression in androgen-treated prostate cancer cell. TMEPAI is highly expressed in many tumor cells. However, little is known about the transcriptional mechanism regulating TMEPAI gene expression, and its promoter has not yet been characterized. In this study, the 5' regulatory region of the TMEPAI gene was characterized, the 5' flanking sequence of TMEPAI gene was successfully amplified by PCR, and the 925 bp fragment of TMEPAI gene promoter was inserted into pGL4-Basic vector to measure its activity. Furthermore, the transcriptional regulators in TMEPAI promoter region were analyzed by bioinformatics. Taken together, these results will help to understand the expression regulation of TMEPAI and its role in tumorigenesis.