Cytocompatibility Comparative Study of Hydroxyapatite Coating on Titanium Alloy Treated with two Chemical Routes

摘要

In order to prepare bioactive hydroxyapatite (Ca_10 (PO_4)_6(OH)_2, HA) coating with ideal biocompatibility, the surface of titanium alloy was treated with the two kinds of chemical methods, which are the acid-alkali-combination method and Self-polymerization-adhesion of dopamine. After pretreatment, the treated titanium alloy plates were immersed in simulated body fluid (SBF) to form HA coating on their surface. The chemical composition of the coating was analyzed by an X-ray diffraction (XRD) and the morphology was observed by a scanning electron microscope (SEM). After that, the plates were training in vitro cytotoxicity test with MC3T3-E1 osteoblasts. Compared with the results of cell culture, the method of Self-polymerization-adhesion of dopamine showed better cell adhesion and proliferation.