Layer-by-layer Assembled Multilayer Membrane with Controllable Amount of Growth Factors for Osteogenic Differentiation

摘要

Creating a bioactive cell-microenvironment and osteogenic functionality interface can be used for the surface modification of bone tissue engineering material, showing a good application prospect. In this paper, a bioactive and osteogenic functionality multilayer membrane with controllable amount of growth factors was fabricated by layer-by-layer assembly technique. Four biological macromolecules (casein, heparin, aidic fibroblast growth factor (aFGF) and collage I) were introduced onto the surface of polyurethane (PU) membrane. Fluores-cence intensity analysis and a toluidine blue assay revealed that the successfully multilayer membrane assembly process. The amount of aFGF loaded on membrane was regulated by changing the concentration of heparin, and aFGF can sustainably released from the membrane for 8 days. FDA staining and MTT assay showed that mesen-chymal stem cells (hMSCs) on growth factors multilayer membrane fully spread and displayed more obvious proliferation behavior. These preliminary results collectively show a practical and simple assembly technique, through which a bioactive and osteogenic material interface can be easily fabricated. This setup has the potential to be used for bone tissue engineering.