The Effect of Leaf Biopesticide Mirabilis jalapa and Fungi Metarhizium anisopliae to Immune Response and Mortality of Spodoptera exigua Instar IV

摘要

Spodoptera exigua is one of insect causing damage in agriculture sector. This insect can be controlled by a natural biopesticide by combining two agents of biological control, biopesticides Mirabilis jalapa and entomopathogenic fungi Metarhizium anisopliae, considered to be virulent toward a wide range of insects. The objective of research was to determine the effect of biopesticides M. jalapa and fungi M. anisopliae against immune system and mortality of S. exigua. This research used a complete randomized block design with five concentrations Mirabilis jalapa and optimum dose of M. anisopliae. A high dose of M. jalapa (0.8% w/v) is the most effective one to decrease total haemocytes especially granulocyt and plasmatocyt (cellular immune) and decrease the concentration of lectin (humoral immune) from S. exigua (p< 0.05). The combination of M. jalapa (0, 8% w/v) and lethal dose of M. anisopliae 2.59 x 107 spore/ml were significant to increase mortality of S. exigua within 48 hours (p< 0.05).