Synthesis, Characterization and In Vitro Evaluation of Strontium- Containing Sol-gel Derived Bioactive Glass/Biphasic Calcium Phosphate Nanocomposite

摘要

In the present research, strontium containing nano-bioactive glass (NBG-Sr) was synthesized by sol-gel method. The morphology was analyzed by transmission electron microscope (TEM). Different amounts (0.5 to 5 wt%) of NBG-Sr were then added to biphasic calcium phosphate (BCP). They were sintered at different temperatures, i.e., 1100, 1200 and 1300°C and changes in physical and mechanical properties were investigated. A sharp decrease in pore volume was observed as the temperature increased. The maximum bending strength (～45 MPa) was achieved for BCP which was mixed with 3 wt% NBG-Sr and sintered at 1200°C. This value was approximately the same when it was sintered at 1300°C. The bending strength failed when both lower and higher amounts of 3 wt% NBG-Sr were utilized. Therefore, sintering of composites at 1200°C was economically reasonable. The X-ray results showed that NBG-Sr additive did not change the phase composition of BCP when it was heat treated at 1200°C. The attachment and proliferation of rat calvarium-derived osteoblasts on samples sintered at 1200°C were also evaluated by scanning electron microscopy (SEM). Based on cell studies, all NBG-Sr-added BCPs supported attachment and proliferation of osteoblastic cells. Overall, biphasic calcium phosphate materials with improved mechanical and biological properties can be produced by using certain quantity of strontium-containing bioactive glass particles.