Determination of tannins content in banana stalk and its silage

摘要

Tannins content is one of the main measurement indexes of feedstuff from banana stalks. This study aims to develop a spectrophotometric method to determine tannins content of banana stalk and its silage. The tannins in banana stalk and its silage were extracted by boiling water bath. Using sodium tungstate-phosphomolybdic acid solution as color-developing agent, after 60 min of developing time, the absorbance at 765 nm wavelength was determined, and verified the linear relation between tannins content (C) and absorbance at 765 nm (A) (C=10.930×A, R~2=0.9992). The credibility of the method was evaluated by determining detection limit, precision and accuracy. The evaluation demonstrated the spectrophotometric method with sodium tungstate- phosphomolybdic acid is feasible and satisfactory for determination of tannins content in banana stalk and its silage.