Domestication and Screening of Saccharomyces Cerevisiae Strain Resistant to Inhibitors in Lignocellulosic Hydrolysates by Acclimatizing Inhibitory

摘要

In order to find the strains which can produce high ethanol yield as well as tolerate inhibitors on the lignocellulosic hydrolysates for developing the renewable bioenergy, the sepecial yeast must be explored. After acclimatizing 23 days and using five different acclimation media with sequential increase in the concentration of inhibitory compounds, a kind of saccharomyces cerevisiae strain resistant to inhibitors was obtained. When the yeast resistant to drug and the parent strain grew in the same media which contained several inhibitory compounds 3.2 g/L acetic acid, 0.8 g/L furfural, 0.4 g/L formic acid, the new yeast's maximal ethanol yield can reach 0.428 g/g, up to 85.6% of theoretical ethanol yield. Compared with drug resistant yeast, the parent strain's maximal ethanol production yield only can reach 0.246 g/g, up to 52.8% of theoretical ethanol yield. After 5 continuous ages, the average ability of producing ethanol was stable. Compared with parent strain, the yeast resistant to drug had good ability to ferment glucose and produce ethanol as well as tolerate inhibitors. The new yeast has extensive application prospect in the bioethanol production.