Determination of polycyclic aromatic hydrocarbons (PAHs) in Ginkgo biloba extracts by HPLC with fluorescence detection

摘要

A rapid extraction method involving ultrasound wave and microwave synergistic extraction (UMSE), followed by sample clean-up on a neutral Al_2O_3 cartridge, high performance liquid chromatography (HPLC) with fluorescence detection, was optimized for polycyclic aromatic hydrocarbons (PAHs) determination in Ginkgo biloba extracts (GBE). The optimum extraction conditions of UMSE were carried out with 5 g of GBE and 40 mL n-hexane/dichloromethane (1:1, v/v), on ultrasound wave and microwave simultaneous mode of working for 9 min for one time. The extraction mean recovery of total PAHs amounted to 88.69%. The optimized procedure, that presented good analytical performances could be applied to determine PAH content in GBE.