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Toxicity of As on Soil Neutral Phosphatase

机译:毒性与土壤中性磷酸酶

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摘要

Toxicity of As on soil neutral phosphatase was studied through a series indoor incubation experiments. Results showed that the activity of soil neutral phosphatase was significantly inhibited by As pollution, and the peak inhibiting ratio was appeared at the sixth incubation day. While the activity of soil neutral phosphatase was also decreased with concentration of As increasing in the same incubation day. The relationship between the concentration of as and soil neutral phosphatase was fit by model ①y=c/(1+bx) and ②y=c(1+ax)(1+bx). The model ② was more suitable to describe the relationship between the concentration of As and soil enzyme. This means the effect mechanism of As on the soil neutral phosphatase was part of inhibition. The Km of neutral phosphatase was increase by As concentration adding, while the V_(max) was lowered. This kind of inhibition belongs to mix competition inhibition.
机译:通过系列室内培养实验研究了如土壤中性磷酸酶的毒性。结果表明,污染,土壤中性磷酸酶的活性显着抑制,第六次孵育日出现了峰值抑制率。虽然在相同培养日内,土壤中性磷酸酶的活性也随浓度的增加而降低。通过模型适合AS和土壤中性磷酸酶的浓度与= C /(1 + Bx)和= C(1 + AX)(1 + Bx)的关系。模型②更适合描述AS和土壤浓度之间的关系。这意味着如土壤中性磷酸酶的效果机制是抑制的一部分。通过浓度加入增加中性磷酸酶的Km,而V_(MAX)降低。这种抑制属于混合竞争抑制。

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