Cytological Analysis of the Effect of Gametocidal Chromosome 2C on ChineseSpring-E. elongatum 7E Disomic Addition

摘要

Elytrigia Desv is an important parent plant in wheat breeding because of its good characters. In this study, according to the complete or incomplete gametocidal effect, ChineseSpring-Ae.cylindrical-2C disomic addition were used to cross with ChineseSpring-E. elongatum (E. elongatum, 2n=14EE) 7E disomic addition. The chromosome aberration were induced and the translocation of wheat-E. elongatum 7E were created. By observing the Fl and BF1 meiosis of pollen mother cell, it showed that the number of univalents, rod bivalents , multivalents were more than theoretical value. In meiosis metaphase I of CE-7E" X CS-2C", the configuration of chromosome pairing was 3.88 I +16.18 II +0.51FII+0.23 IV Even in anaphase I II, there were a lot of abnormal phenomena, such as lagging chromosomes and fragments. By using C-banding, the materials with translocation were identified. After checked 102 plants of F2, we obtained 7 translocation plants, including 1 inter-wheat-genome translocation. The translocation frequency was 6.86%.