Characterization of putative a (1,3)-β-D-glucan (curdlan) synthase for a low molecular weight curdlan biosynthesis from Agrobacterium sp. M503

摘要

A β-(1,3)-D-glucan (curdlan) synthase gene for a low molecular weight curdlan biosynthesis, crdS_(Ag), from Agrobacterium sp. M503 was cloned and its encoding protein was characterized by several online protein analysis softwares. The crdS_(Ag) consists of 1965-base-pairs Open Reading Frame (ORF) encoding a protein with molecular weight approximate 73.5 kDa, which contains the conserved domain of CESA-CelA_like belonging to glycosyltransferase family 2 (GT2). Moreover, CrdS_(Ag) was a membrane protein with seven hydrophobic transmembrance domains. The second structure analysis indicated it was composed of 43.12% α-helix, 17.89% β-sheet, and 38.99% random coil structure. These data will lay a foundation to clarify the biosynthesis mechanism of the low molecular weight curdlan.