The Effects of Enzyme to the Dissociation of Cells in Monolayer and 3D Microtissue on the Liquid Crystal Substrate

摘要

The technique first developed by our research group to culture three dimensions microtissues using liquid crystal substrate has potential to be used for cytochemical study. In order to demonstrate the differences in cell response to the cytochemical treatments, this paper applied an enzyme as a model drug to compare the enzymatic dissociation of tells grown in monolayer on a culture dish and microtissues cultured on the liquid crystal substrate. The results showed that the cells were fully dissociated layer by layer at a time course of 90 minutes. The monolayer of cells was dissociated directly by trypsinization within 6 minutes. Obviously, cells embedded deep in the microtissues were encapsulated or well protected from the treatment of EDTA-trypsin and this led to longer period of enzymatic dissociation.