Producing of Microalgal Lipid by Isolated Microalgae under Photoautotrophic and Heterotrophic Cultivations

摘要

The objective of this work is to produce lipid from isolated freshwater microalgae strains. Under photoautotrophic cultivation, a biomass of 1.67g/L with lipid of 23.94mg/L, 1.2g/L with lipid of 37.0mg/L, 1.13g/L with lipid of 36.4mg/L were obtained from microalgae isolate Chlorella sp. MSU1, Chlorella sp. MSU2 and Chlorella sp. MSU3, respectively. A biomass of 2.33g/L with lipid of 94.9mg/L, 2.73g/L with 398.9mg/L, 5.28g/L with lipid of 67.0mg/L were found from Chlorella sp. MSU1, Chlorella sp. MSU2 and Chlorella sp. MSU3, respectively, under heterotrophic condition. Heterotrophic lipid production of microalgae Chlorella sp. MSU2 reached the maximum of 797.7mg/L at 40 g/L glucose was obtained. Maximum cell yield coefficient (Y_(x/s)) was found of 0.733 using 20 g/L glucose, whereas volumetric lipid production rate (Q_p) of 132.9mg/L/d was obtained using 40g/L glucose.