Comparison between Dextran-coated Superparamagnetic Iron Oxide Nanoparticles and Magnetoliposome on Rabbit Corneal Endothelial Cells Labeling

摘要

Purpose: To evaluate and compare the promising applications of dextran-coated superparamagnetic iron oxide nanoparticle (dSPION) and magnetoliposome on corneal endothelial cells transplant therapy. Methods: Two kinds of novel superparamagnetic iron oxide nanoparticles (SPIONs), dSPION and magnetoliposome were synthesized, characterized and incorporated to rabbit corneal endothelial cells (RCECs). Uptakes of SPIONs were identified by Prussian blue staining. Cells migrating rates in magnetic field were calculated. Cytotoxicities of the two SPIONs were detected using MTT assay. Result: Magrating rates of dSPION (4～64 μg/mL) labled RCECs were 24.1±2.4%～78.3±2.2%, and 5.0±1.7% of unlabeled RCECs. (p<0.05) Magrating rates of magnetoliposome (16～265 μg/mL) labeled RCECs were 39.6±4.2%～83.8±6.5%, and 6.9±1.0% of unlabeled RCECs. (p<0.05) The absorbance values of aSPION (4～4 μg/mL) labled RCECs in MTT assay were 0.317±0.017 ~ 0.328±0.025. The absorbance values of magnetoliposome (16～265 μg/mL) labled RCECs were 0.319±0.015～0.331±0.022. There were no significant variation between all mentioned absorbance values and that of unlabeled RCECs. (p>0.05) Conclusion: RCECs labeled with each SPION migrated significantly in external magnetic field, while cytotoxicities did not revealed. Our study demonstrated the potential applications of dSPION and magnetoliposome on labeling RCECs for cell transplantation.