Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus during freeze-drying

摘要

Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus was studied by counting viable cells before and after freeze-drying. The concentrations of five carbohydrates were all 3%, 6%, 9%, 12% and 15%. Results showed as follows: the optimal concentrations of trehalose, soluble starch, raffinose and galactose were 12%, 9%, 3% and 15%, the survival of Lactobacillus acidophilus was 42.62%, 56.50%, 45.05% and 42.69% and the viable cells of lyophilized powder were 0.40×10~(11)cfu/g, 0.45×10~(11)cfu/g, 0.52×10~(11) and 1.09×10~(11)cfu/g, respectively.