Synthesis and characterization of CD44 targeted nanoparticle PEI/pDNA/HA-mPEG for gene delivery

摘要

In order to prepare targeted nanocarrier to gene delivery, a novel nanoparticle was designed to condense pDNA. Hyaluronic acid was regarded as the targeted bio-materials because the HA-receptor-mediated CD44 receptors existed in many issues which were interacted with tumor membrane. The preparation procedure was determined by N/COOH and condensing pDNA capability was measured with agarose gel electrophoresis assay. The HA-mPEG polymer was characterized by FI-IR and PEI/pDNA/HA-mPEG nanoparticle was characterized by Nano Zetasizer. The obtained results was showed that the HA-mPEG was successfully synthesized and the ternary complexes presented relatively uniform particle distribution with 200-450 nm, the zeta potential was increased with the increasing content of PEI. The PEI/DNA/HA-mPEG nanoparticle showed the potential in gene delivery.