Preparation and Characterization of Metronidazole liposome by Supercritical Reverse Phase Evaporation Method

摘要

Supercritical reverse phase evaporation (SRPE) is a new technique for preparation liposomes with supercritical fluid instead of organic solvent. The process has characteristic for simple and a high coating ratio for water-soluble substances. In this paper, the technique was used to synthesize metronidazole liposomes and the effects of the process parameters on the particle size and the coating ratio of liposomes were investigated in detail. The liposomes with a volume average diameter of 190-350nm were successfully prepared by this technique and the coating ratio for metronidazole liposomes was as high as 48.2%. It was confirmed that the temperature, pressure and equilibrium time had great effects on the particle size and the coating ratio.As the temperature, pressure increased and the solution pH increased from 1 to 7, the particle size increased to a maximum and then gradually decreased, while the coating ratio increased to the maximum and then showed a significant downward trend, indicating that the rate of metronidazole liposomes coated was more susceptible to the influence of the experimental parameters.