Adaptive Electrical Stimulation to Improve In-Vitro Cell Growth

摘要

An adaptive system is used to stimulate electrically (In-Vitro) muscle vagina cells and epithelial rabbit bladder cells with external electrical stimulation ES. Waveform stimulation is synthesized from both: A specific known action potential or by a set of independent ionic potentials conforming a multi output system. Each ionic potential is the output of a set of FIR filters in which the coefficient adaptation is carried out by using a time varying step size normalized LMS (NLSM) algorithm. The adaptive system output stimulates in-vitro culture cells emulating biological neurotransmitters action. Electrical stimulation (ES) shows that cells under test grow faster than non-stimulated ones, observing bigger cell viability in stimulated cells than non stimulated. The objective of the experiment is to analyze the effect of external electrical stimulation ES on confluence and cell viability in culture cells by MTT (3-[4,5-Dimethylthiazol-2-Yl]-2,5-diphenyl-tetrazolium bromide) assays.