Isolation of oxymatrine from alkaloids of Sophora flavescens Ait. by H103 macroporous resin

摘要

An efficient and environment-friendly macroporous resin column chromatographic separation method has been developed for preparative isolation of oxymatrine from alkaloids of Sophora flavescens Ait. in the present study. The static adsorption ratios of nine macroporous resins were evaluated. The results demonstrate that H103 macroporous resin, showing high adsorption and desorption capacity, is suitable for separation of oxymatrine. The optimal parameters are obtained: the H103 resins are packed with ethanol, height-diameter ratio (H/D in short) of H103 resin-packed chromatogram column is 33:1, the elution program is 30% C_2H_5OH-25% NH_4OH (115:1, v/v) at flow rate of 2.0 mL/min. After isolation on resin column chromatography, target compound is adequately separated from interferences; the purity of oxymatrine is increased to 91.53% by GC-MS analysis and the recovery yield of oxymatrine is 78.19%.