Analysis of 2,6-Dinitrotoluene Toxicity to Common Carp (Cyprinus carpio)

摘要

2,6-dinitrotoluene (2,6-DNT) is the preferential toxicants stipulated by OECD and EPA of USA. The present study was conducted to determine the effect of 2,6-DNT chemical on acute toxicity and subacute toxicity in common carp (Cyprinus carpio). The 24h, 48h, 72h and 96h LC50 values of common carp by 2,6-DNT were 1.137±0.023mg/L, 0.830±0.024mg/L, 0.661±0.019mg/L and 0.479±0.020mg/L, respectively. The subacute experiment shows that C. carpio are rarely affected by 0.0251mg/L 2,6-DNT, but significantly affected by 0.0398, 0.0631, 0.0794mg/L 2,6-DNT in contrast to the controlled sample (p < 0.05). The accumulative rate (K) is 1.25 for C. carpio exposed to 2,6-DNT. This shows that there are significant accumulative actions in common carp. Among 0.024 - 0.420mg/L 2,6-DNT, the higher the 2,6-DNT concentration, the stronger the Na~+/K~+-ATPase are inhibited. The IC50 values (the concentration of 2,6-DNT required to effect 50% inhibition of Na~+/K~+-ATPase) following gill, kidney and liver on C. carpio are 0.18mg/L, 0.24mg/L and 0.23 mg/L, respectively. The toxicity order of the observed IC50 values of 2,6-DNT to C. carpio tissues has been found as following: gill> liver > kidney.