Molecular Cloning and Characterization Analysis of 3,8-Divinyl protochlorophyllide a 8-vinyl Reductase Gene from Dunaliella parva

摘要

The vast majority of photosynthetic organisms utilize monovinyl chlorophyll for their photosynthetic reactions. For the biosynthesis of monovinyl chlorophyll, the reduction of the 8-vinyl group which is located on the B-ring of the macrocycle is essential. 3,8-Divinyl protochlorophyllide a 8-vinyl reductase (DVR) catalyzes the reduction of 8-vinyl group on the tetrapyrrole to an ethyl group, which is necessary for monovinyl chlorophyll (Chl) synthesis. The former studies indicated the DVR could enhance photosynthesis. The full-length cDNA encoding DVR was obtained from oleaginous microalgae Dunaliella parva, which include 1326 bp open reading frame (ORF), 22 bp 5'-untranslated sequence and 383 bp 3'-untranslated sequence. Dunaliella parva DVR showed the highest sequence similarity with the DVR from Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva DVR also showed wide similarity with other species.