Fast degradation of chlorpyrifos in soil by strain Cupriavidus taiwanensis X1 labeled with luxAB

摘要

For researching ecological behaviors of Cupriavidus taiwanensis XI which has strong hydrolysis activity on chlorpyrifos(CP), reporter gene luxAB was successfully introduced into cells by electroporation. The labeled strain XI -lux with genetic stability and fluorescence was obtained. Both of strain XI and XI -lux could completely degrade 200 mg/1 CP within 12h in minimal salt medium, and experimental results showed that introduction of luxAB did not affect strain XI growth and degradation on CP. The cells of X1-lux and XI were inoculated into soil with 500 mg/1 CP, the cell concentration and CP residual was detected, and data revealed that strain XI could absolutely removed CP in 12d and survive in soil. Strain XI is a potential excellent choice for bioremediation of organophosphorus polluted environments.