Protection against UVB-induced Oxidative Stress in Human Skin Cells and Skin Models by Methionine Sulfoxide Reductase A

摘要

Environmental trauma to human skin can lead to oxidative damage of proteins and affect their activity and structure. When methionine becomes oxidized to its sulfoxide form, methionine sulfoxide reductase A (MSRA) will reduce it back to methionine. We report here the increase in MSRA in normal human epidermal keratinocytes (NHEK) after ultraviolet B (UVB) radiation, as well as the reduction in hydrogen peroxide levels in NHEK pre-treated with MSRA after exposure. Further, when NHEK were pre-treated with a non-cytotoxic pentapeptide containing methionine sulfoxide, MSRA expression increased by 18.2%. Additionally, when the media of skin models were supplemented with the methionine sulfoxide pentapeptide and then exposed to UVB, a 31.1% decrease in the production of sunburn cells was measured. We conclude that MSRA contributes to the protection of proteins against UVB-induced damage and that pre-treatment with a methionine sulfoxide-containing peptide reduces oxidative damage in NHEK and skin models.