Research the Equilibrium Distribution of Puerarin in Aqueous Two-phase Combine with Temperature-induced Phase Separation EOPO/ K_2HPO_4 /Water Systems

摘要

The partition behavior of Puerarin in ethylene oxide-propylene oxide random copolymer(EOPO)/ K_2HPO_4/water systems was investigated .The optimum conditions were EOPO (SDP30) 28%, K_2HPO_4 24%, temperature-induced condition was 70°C and 30min. yield of Puerarin could reach 42.26%. Based on the modified model lnK/R2=A*+b*(w"-w')+c*(w"-w')2, partition coefficients of Puerarin in EOPO/K_2HPO_4 aqueous two-phase systems were correlated. It was found that the correlation of the experimental data was quite satisfactory.