Cloning and Sequence Analysis of Two cDNA Encoding Invertase Inhibitors from Cassava (Manihot esculenta Crantz)

摘要

In this study, two encoding invertase inhibitors (INH) named as MelNHl and MeINH2 were isolated from cassava {Manihot esculenta Crantz). The MelNHl contains a 583 bp fragment in length and codes a region of 172 amino acids; and the MeINH2 contains a 538 bp fragment in length and codes a region of 169 amino acids. The amino acid sequence analysis showed that both MelNHl and MeINH2 have typical structure of invertase inhibitor proteins with a N-terminal signal peptide, four conserved cysteine residues and two disulfide bridges, respectively; whereas, they showed low homology to other organisms. This research provides a foundation for further study of the cassava INH.