Study on Pure Aqueous Extraction Method of PHB/Ectoine Co-products Based on Osmotic Downshock

摘要

The simultaneously co-production of Poly-P-hydroxybutyrate (PHB) and ectoine in a process (PHB/Ect co-production) and co-products extraction have great significant for reducing the manufacture cost and promoting industrialization of PHB and ectoine. The pure aqueous extraction method based on osmotic downshock was used for the extraction of PHB/Ect co-products by H. salina. The effects of osmotic pressure, extraction temperature and extraction time on the extraction rate of PHB were investigated. The ectoine was extracted and purified by the techniques of hollow fiber and ion exchange. The optimal conditions for PHB extraction were osmotic downshock in pure water, extraction temperature at 60 °C and extraction for 4 h. The extraction rate of PHB was 87.5%. The extraction rate of ectoine was 84.2%.