The Enzymatic Action of Cellulase on Pigment-dyed Cotton Fabrics

摘要

In this research, cotton fabrics were colored with a pigment suspension, and then treated with an acid cellulase to investigate the influences of pre-existing pigment on cellulase action. It was found that the pigment on cotton surface could increase the affinity between cotton and cellulase protein due to the mutual attraction between pigment molecule and non-polar residues in the enzyme. However, it was observed that the existing pigment would suppress enzymatic activity by testing the soluble reducing sugars liberated during the treatment. The main reason is that the pigment could reduce the accessibility of cellulase protein to the cotton molecular chains by steric hindrance.