Different inoculation methods were employed in the process of the Cordyceps militaris subculture. The experimental results indicated that fruit-body yields of strains obtained by mycelium subculture using transfer needle were unstable, and the subcultures obtained by transferring mycelial cakes made by using a punching bear had the relatively high stability. Two kind of subcultures had a significant difference in fruit-body yield using one-way ANOVA analysis (P<0.05).
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