The differential diagnostic model for asymptomatic HBV carriers established by MALDI-TOF-MS analysis

摘要

Objectives: Hepatitis B virus can result in asymptomatic carrier (AsC) state or chronic inflammation of liver, which depends on the host immunity. The peptidomic profilings of HBV infection and immune responses are not thoroughly known for us. Methods: hi this study we established the diagnostic model and searched for the specific peptides which may influence various outcomes of HBV infection and could be used as clinical indicators for outcomes of HBV infection. Serum from 116 HBV infected (AsC and chronic hepatitis), 60 HBV-immunized and 70 normal subjects were treated with magnetic bead-based MB-WCX kits and analyzed by the Clinprot/ Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) techniques. Purified plasma proteins were subjected to FT-ICR-MS analysis. Results: The serum peptide profiling had changed in asymptomatic carrier (AsC) and aggregately in chronic hepatitis. The specific model comprised of two peptides m/z 2882.89 and 4476.12 could distinguish HBV infected from healthy (HBV-immunized and normal) group and showed 95.5% of sensitivity and 95.4% of specificity by cross-validation analysis. 40/ 56 HBV infected and 43/ 50 healthy subjects could be correctly classified by the model. The area under the receiving operating curves of m/z 2882.89 and 4476.12 which were identified as fibrinogen beta chain (FBG) and nucleophosmin (NPM) respectively were both up to 0.88 when discriminating AsC from the healthy group. Conclusions: There was specific serum peptidomic profiling in the serum of HBV infected patients, and m/z 2882.89 and 4476.12 could be valuable follow-up and prognostic tools for HBV infection.