Effect of MgSO_4, NaHCO_3, KH_2PO_4, K_2HPO_4and ascorbic acid on survival of Bifidobacterium bifidum during freeze-drying

摘要

Effect of MgSO_4;;NaHCO_3;;KH_2PO_4;;K_2HPO_4 and ascorbic acid on the survival of Bifidobacterium bifidum and the number of viable cells of lyophilized powder was studied. Results were as follows: addition of MgSO_4, NaHCO_3, KH_2PO_4, K_2HPO_4 and ascorbic acid into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of MgSO_4, NaHCO_3, KH_2PO_4 , K_2HPO_4 and ascorbic acid were 0.20%, 0.60%, 1.80%, 1.40% and 2.20, the survival of B. bifidum was 78.31%, 77.92%, 77.80% ,79.82% and 71.62, respectively.