Ultrasonic extraction for obtaining IGF-1 from antler velvet residue and chemical compositions

摘要

In this work, the utilization of ultrasonic technology to extract insulin like growth factor (IGF-1) from antler velvet residue was evaluated. The effects of pH of solvent, the ratio of solvent to sample, extraction temperature and extraction time were investigated through a single factor experiment. The influences of preliminary conditions of supercritical CO_2 and co-solvent extraction (SFE) on the activity remaining of IGF-1 in antler velvet residue were discussed. The contents of total protein, total acidic polysaccharide and amino acids were determined, respectively. The results indicate that SFE method has slightly influence on the activity remaining of IGF-1 in antler velvet residue, and the activity remaining of IGF-1 in residue reached 93.68 %. In the optimal pretreatment condition (pHIO buffer solution; the ratio of solvent to sample 20/1 (v/w); extraction temperature 0 °C-35 °C; extraction time 4×15 min), the process yielded 8006.05 ng of IGF-1 per gram antler velvet residue. While the contents of total protein and total acidic polysaccharide were 107.31mg/100mL and 800.7ug/100mL, respectively. Moreover, the total amino acid (TAA) content was up to 120.44mg/100mL and 7 kinds of essential amino acid were 30.08% of the TAA.