Physical Optics Corporation (POC) presents a novel Mobile ELISA-based Pathogen Detection system that is based on a disposable microfluidic chip for multiple-threat detection and a highly sensitive portable microfluidic fluorescence measurement unit that also controls the flow of samples and reagents through the microfluidic channels of the chip. The fluorescence detection subsystem is composed of a commercial 635-nm diode laser, an avalanche photodiode (APD) that measures fluorescence, and three filtering mirrors that provide more than 100 dB of excitation line suppression in the signal detection channel. Special techniques to suppress the fluorescence and scattering background allow optimizing the dynamic range for a compact package. Concentrations below 100 ng/mL can be reliably identified. The entire instrument is powered using a USB port of a notebook PC and operates as a plug-and-play human-interface device, resulting in a truly peripheral biosensor. The operation of the system is fully automated, with minimal user intervention through the detection process. The resolved challenges of the design and implementation are presented in detail in this publication.
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