Simultaneous Determination of Catecholamines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence Detection

摘要

A micellar electrokinetic chromatography method with laser-induced fluorescence detection was developed to determination catecholamines (dopamine, norepinephrine and epinephrine) after derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein. The baseline separation was achieved within 18 min with running buffer composed of 30 mM sodium borate (pH 9.0), 40 mM sodium cholate, 4.0% acetonitrile (v/v), and applied voltage 25 kV. The detection limits for norepinephrine, epinephrine, and dopamine were 0.35 ng/mL, 0.025 ng/mL, and 0.20 ng/mL. The micellar electrokinetic chromatography method was applied to determinate catecholamines in urine samples.