A pre-column derivatization-HPLC method for determination of oxalipIatin(L-OHP)content and entrapment efficiency in nanoparticles

摘要

Purpose: To develop an improved -HPLC method for the determination of oxaliplatin(L-OHP) content and entrapment efficiency in nanoparticles. Methods: After derivatization with sodium diethyldithiocarbamate (DDTC), Pt(DDTC)2 and diazepam (the internal standard) was separated on C18 column (250 mm×4.6 mm, 5 urn) at 30°Cusing a mobile phase consisting of methol and water (75:25, v/v) at flow rate of 1 ml-min-1; The method was validated with respect to selectivity, linearity, precision and accuracy, limit of quantification and stability. The ultrafiltration was employed to separate the free L-OHP from nanoparticles to calculate the entrapment efficiency. Results: The calibration curve of L-OHP was linear in the concentration range of 5.26-31.53 ugml-1. (r= 0.9997) with the limit of quantification (LOQ) of 0.42 ug/mL. The variation coefficients were not more than 1.63%. The mean recoveries was from 98.29% to 100.45%. The content and the encapsulation efficiency of L-OHP nanoparticles were 1.69mg/ml and 63%, respectively. Conclusion:The present method can be used to determine L-OHP content in prepared nanoparticles.as well as entrapment efficiency.