Short thio-multi-walled carbon nanotubes and Au nanoparticlesenhanced electrochemical DNA biosensor for DNA hybridizationdetection

摘要

A novel and sensitive electrochemical DNA biosensor based on multi-walled carbon nanotubes functionalized with a thio group (MWNTs-SH) and gold nanoparticles (GNPs) for covalent DNA immobilization and enhanced hybridization detection is described. The key step for developing this novel DNA biosensor is to cut the pristine MWNT into short and generate lots of active sites simultaneously. With this approach, the target DNA could be quantified in a linear range from 8.5 x 10~(-10) to 1.5 x 10~(-5) mol/L, with a detection limit of 1.67x 10~(-11) mol/L by 3σ.