Preparation and Characterization of A Novel Silica Fluorescent Nanoparticles with DPPDA-Eu~(3+) doped

摘要

A new ligand of 4,7-Diphenyl-1,10-phenanthroline-2,9-dicarboxylic acid (DPPDA) and Eu~(3+)chelate compound of this ligand were prepared. Then DPPDA-Eu~(3+) doped silica fluorescent nanoparticles of DPPDA-Eu~(3+)/SiO_2 with primary amino groups on their surface were developed using a water-in-oil (W/O) microemulsion technique. Characterizations by transmission electron microscopy, fluorescent spectra, fluorescent molecules leaking experiments and photostable experiments show that the nanoparticles are spherical and uniform in size of 80±8nm, monodisperse, highly fluorescent signal and highly photostability. No obvious of fluorescent molecules leaking was observed. The nanoparticle as a novel fluorescent probe is expected to be applied in highly sensitive bioassays such as time-resolved fluorescence, biosensor and biochip.