Nove fluorescent eruopium chelate-doped silica nanoparticles:preparation,characterization and time-resolved fluorometric application

摘要

Novel fluorescent europium(III)chelate-doped silica nanoparticles wre prepared and characterized as a new type of fluorescence probe for quantitative bioassay.The preparation was carried out in a water-in-oil(w/o) microemulsion consisting of a strongly fluorescent Eu~(3+)(BHHT-Eu~(3+)),surfactnat (Triton X-100),co-surfactant (n-hexanol,n-hexanedion-6"-yl)-omicron)terphenyl-Eu~(3+)(BHHT-Eu~(3+)),surfactant (Triton X-100),co-surfactant(n-hexanol,hydrolysis of tetraethylorthosilicate(TEOS).The effects of different co-surfacohexane)by controlling the hydrolysis of tetraethylorthosilicate (TEOS).The effects of different co-surfactants and aqueous phases on the size and fluorescence lifetime of the nanoparticles were investigated.The results reveal that the size of the size and fluorescence life tim eof the nanoparticles were investigated.The results reveal that the size of the nanoparticles is decreased with a change of co-surfactants from -hexanol to n-octanol,and the fluorescence lifetime of the nanoparticles is surface modification and bioconjugation of the nanoparticles.Nanoparticle-labeled streptavidin(SA)was used for the time-resolved fluoroimmunoassay of human hepatitis B surface antigen (HBsAg).The result shows that the new fluorescent europium(III0chelate-doped silica nanojparticles are suitable to be used as a fluorescence probe for highly sensitive bioassays.