Analysis of Changes of Ca Concentration and Mitochondrial Membrane Potential in HepG-2 Induced by CSBE by Laser Scanning Confocal Technology and Flow Cytometry

摘要

To discuss the mechanism of the apoptosis induced by n-butanol extract from Capparis spionosa L. (CSBE) in human heptocarcinoma cell Line HepG-2, Laser Scanning Confocal Technology and Flow Cytometry were used to investigat the changes of mitochondrial membrane potential and Ca~(2+) levels in HepG-2 cells. The changes of mitochondria membrane potential induced by CSBE on the HepG-2 cells were studied by flowcytometry. Intracellular Ca~(2+) levels of both the treated and untreated HepG-2 cells with CSBE were measured by laser confocal microscope. The mitochondria membrane potential of HepG-2 cells decreased in various degrees under CSBE. In addition, the intracellular Ca~(2+) level increased in the middle and high dose groups, resulting in an imbalance. CSBE can decrease the mitochondria membrane potential, open MPTP channel and cause the overload of intracellular Ca~(2+) level, which may induce the apoptosis of HepG-2 cell. These instructions give you basic guidelines for preparing camera-ready papers for conference proceedings.